Absolutely! Barcelona, Madrid, and Toledo are beautiful Spanish cities, each with its distinct flair. Here are some tips to enrich your travel experience:

Best Time to Visit:
For pleasant weather and fewer crowds, the best times to visit Spain are during the spring (April to June) and fall (September to November). July and August can be crowded and hot.

Route and Accessibility:
All three cities are well connected by high-speed trains. Start from Barcelona, which is well connected internationally, move to Madrid through a 3-hour high-speed train journey, and from there you can reach Toledo which is just a 30-minute train ride away.

Altitude and Acclimatization:
The altitude in these places isn't high, so acclimatization is not an issue.

Accommodation and Booking:
It's advisable to book accommodation in advance, especially if your trip is during the tourist season. All these cities offer a range of accommodations, from luxury hotels to budget guesthouses and vacation rentals.

Clothing and Essentials:
Pack light clothing for the summer and layers for the cooler months. Comfortable walking shoes are a must for exploring. Don't forget a hat, sunglasses, and sunscreen for protection from the Mediterranean sun.

Health and Safety:
Stay hydrated and apply sunscreen regularly. Spanish cities are generally safe, but be mindful of pickpockets in crowded tourist areas.

Local Etiquette and Respect:
Spanish people appreciate when travelers greet them and communicate in their local language, even just a few basic phrases. Tipping is not mandatory but appreciated for good service. In Madrid, and especially in Toledo, it's recommended to dress modestly as these are more traditional cities. Remember to be respectful of siesta hours, usually from 2pm-5pm.

Also, in line with the Spanish dining culture, remember that meals often start later than usual, with dinner usually starting around 9 pm and lasting well into the night.